[The role of neuroendocrine mediators in regulatory activity of T-cells].

The aim of the study was to establish the role of some neuroendocrine mediators (agonists and antagonists of ß-adrenergic receptors, progesterone) in regulating T-cells activity. Studies conducted on the culture of leukemiatransformed T-cells (Jurkat cells) (DSMZ-Deutshe Sammulung von Mikroorganismen und Zellkulturen (Germany)). Jurkat Cells (4 x 105 cells/ml) stimulated with 50 µg/ml fitogemaglutinin A (PHA) at 370С for 5 minutes and then incubated for 24 hours alone, or together with ß- adrenergic receptors agonist izopretonolom (at dose of 10-5 M, 10-6 M), an antagonist, propranolol (dose of 10-5 M, 10-6 M) and progesterone (dose 0,07 µl, 0,7 µl) added to the incubation medium. The viability of Jurkat cells was determined by MTT test. It was shown that ß-adlenoretseptors agonist, izoprotenol didn't affect the activity of mitochondrial dehydrogenases in intact and contributed to their low activation (12%) in the mitogen-activated Jurkat cells. ß-adrenergic receptors antagonist, propranolol, promotes a significant reduction in activity of mitochondrial dehydrogenases, and hence the viability of both intact (40-60%) and mitogen stimulated Jurkat cells (20-40%). Viability of intact Jurkat cells didn't change, and dose-dependently increased in PHA-stimulated Jurkat cells during progesterone exposure. It was concluded that viability of the T-cells and hence their functional activity is largely sensitive to the influence of the ß-adrenoceptor antagonists and progesterone. These data should be considered in the clinical application of appropriate drugs.

[Changes of local resistance of oral cavity and humoral immunity among workers of metallurgical and chemical production during parodontitis].

On the basis of the data of immunological investigations of 142 workers of metallurgical (melting shops of Zestaphoni's Farroalloy Plant) and chemical (electrolytic shops of manganese and dioxide manganese of Farroalloy Plant and "Azoti") production it was found that during parodontitis among studied contingent local resistance of mouth cavity and humoral immunity are changed, the compound of lysozyme and amylase in saliva is lowered, in the layers of saliva and blood is revealed the misbalance of immunoglobulin's system. First of all was established, that during parodontitis among the studied workers autoimmune processes are developed directed against the I-st type collagen and the tissue of gum. Changes of local and common homeostasis as well as the changes of intensity of autoimmune process are in direct correlation with the severity of parodontitis and the pollution of production environment with the spray of manganese dioxide.

[Humoral mechanisms of autoimmune process against type I collagen in psoriasis].

Psoriasis represents disease with the involvement autoimmune mechanism against type I collagen, and its degree depends on the form of the disease and its clinical course. There is direct association between the autoantibody titres against type I collagen and the severity of the disease. As developed autoimmune process against type I collagen is specific in traditional treatment process, in spite of definite clinic effectiveness, without usage of specific factors there is no involution in autoimmune process against type I collagen. 134 patients with psoriasis have been investigated. 94 of them had vulgar psoriasis, 20 had erythrodermal form and 20 had arthropatic form. 50 healthy subjects were included in the control group. The age of patients varied from 20 to 70 years. It is confirmed that by treatment using miolophid immunomodulation autoantibody against type I collagen has decreased and treatment was relatively effective. It should be mentioned that despite immunomodulation therapy and achieved effect, there is no complete involution of autoimmune process, as in the case of psoriasis clinical and biological recovery periods do not coincide and thus after discharging from hospital prognosis must be controlled by measuring of autoantibody titres.

[Correlation of local immune system indices of patients with tuberculous and serous meningitis]. / Vzaimosviazi pokazatelei mestnoi immunnoi sistemy bol'nykh tuberkulezom i seroznym meningitom.

The method of discrete-dynamic analysis was used for studying the immunity system state in 60 patients with tuberculous meningitis (TM) of various degree of severity and in 35 patients with serous meningitis of nontuberculous etiology (SMNE). A significant difference was established of the correlation between parameters in the combinations comprising lymphocytes immune to Mycobacterium tuberculosis which enables TM and SMNE differentiation. TM was characterized by a sharp increase in the number of correlations (intensity) in the parameters as compared with SMNE. Intensity significantly declines in the fatal outcome of TM as compared with this parameter in the survived patients. In SMNE local immunity state normalizes (intensity decline) in 1.5-2 months, in TM of moderate severity in 3 months and in a serious course (during the follow-up) intensity does not decline.

[NK cells in patients during complex therapy of destructive pulmonary tuberculosis]. / NK-kletki u bol'nykh v protsesse kompleksnoi protivo-tuberkuleznoi terapii destruktivnym tuberkulezom legkikh.

Functional activity of peripheral blood NK-cells was studied in 85 patients aged 18 to 72 years with active destructive tuberculosis of the lungs. There was detected a significant decrease in the activity of the NK-cells them. The most pronounced decrease was observed in the patients over 50 years. The changes in the functional activity of the NK-cells during chemotherapy correlated with the time course of the tuberculosis process. The results of the study provided the use of the NK-cell activity index in addition to the other indices in estimation of the patients immunity status and control of the treatment efficacy. For correction of immunodeficiency detected by the NK-cell activity early use of immunostimulating agents in the combined therapy of the patients with destructive tuberculosis of the lungs was required.

[Immunologic aspects of the pathogenesis of latent and seroresistant forms of syphilis]. / Immunologicheskie aspekty patogeneza skrytykh i serorezistentnykh form sifilisa.

Studies of the cellular and humoral immunity in patients with latent and seroresistant syphilis have revealed decreased content of T-lymphocytes and elevated levels of B- and antigen-reactive lymphocytes. Reduced T-cell level is due to a decrease of the T-suppressor subpopulation. Unlike latent syphilis, seroresistant form is associated with a complete disappearance of the theophylline-sensitive subpopulation, whereas T-lymphocytes are represented mainly by the theophylline-resistant (helper) subpopulation. Effective therapy of patients with seroresistant syphilis has normalized the counts of T- and B-antigen-reactive lymphocytes, and theophylline-sensitive cells appear in the blood. These data give grounds to prescribe immunostimulating therapy to patients with seroresistant syphilis.